isotype matched control antibody (R&D Systems)
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isotype matched control antibody
Isotype Matched Control Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/igg2b+isotype+control/Mouse+IgG2B+APC-conjugated+Antibody/us12613244-531-21-26
Average 93 stars, based on 103 article reviews
Isotype Matched Control Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/igg2b+isotype+control/Mouse+IgG2B+APC-conjugated+Antibody/us12613244-531-21-26
Average 93 stars, based on 103 article reviews
isotype matched control antibody - by Bioz Stars,
2026-10
93/100 stars
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Cell Culture:Article Title: Overall avidity declines in TCR repertoires during latent CMV but not EBV infection Article Snippet: Ex vivo LILRB1 and CD57 expression along T cell differentiation To assess CD57 and LILRB1 expression along T cell differentiation, CMV/pp65-specific CD8 T cell clonotypes of high versus low avidity isolated and stained as described in the main manuscript (CD8, CD28, CD45RA, CD57, CD85j, CMV/pp65 multimer, Vivid Aqua and appropriate TRBV antibodies (Supplementary Table 1)) were acquired on a Cytoflex flow cytometer (Beckman Coulter) and data were imported in FlowJo software (v.10.4.2, Tree Star) for analysis, using both CD28 and CD45RA as differentiation markers along which LILRB1 and CD57 positive cells were quantified as fractions of parent population. .. CFSE-labelled (Invitrogen) PBMCs from donor BCL4 were pulsed 1 hour at 37°C with native CMV/pp65495-503 peptide (10-8 M) washed and cultured in RPMI 1640 medium (Gibco) supplemented with 8% human serum and 50 U/ml human recombinant IL-2 (GlaxoSmithKline) in the presence of monoclonal IgG2B mouse anti-human LILRB1 (CD85j, ILT-2) blocking antibody or an Recombinant:Article Title: Overall avidity declines in TCR repertoires during latent CMV but not EBV infection Article Snippet: Ex vivo LILRB1 and CD57 expression along T cell differentiation To assess CD57 and LILRB1 expression along T cell differentiation, CMV/pp65-specific CD8 T cell clonotypes of high versus low avidity isolated and stained as described in the main manuscript (CD8, CD28, CD45RA, CD57, CD85j, CMV/pp65 multimer, Vivid Aqua and appropriate TRBV antibodies (Supplementary Table 1)) were acquired on a Cytoflex flow cytometer (Beckman Coulter) and data were imported in FlowJo software (v.10.4.2, Tree Star) for analysis, using both CD28 and CD45RA as differentiation markers along which LILRB1 and CD57 positive cells were quantified as fractions of parent population. .. CFSE-labelled (Invitrogen) PBMCs from donor BCL4 were pulsed 1 hour at 37°C with native CMV/pp65495-503 peptide (10-8 M) washed and cultured in RPMI 1640 medium (Gibco) supplemented with 8% human serum and 50 U/ml human recombinant IL-2 (GlaxoSmithKline) in the presence of monoclonal IgG2B mouse anti-human LILRB1 (CD85j, ILT-2) blocking antibody or an Article Title: Overall avidity declines in TCR repertoires during latent CMV but not EBV infection. Article Snippet: .. In vitro LILRB1 blocking experiments 30-Gy-irradiated HLA-A*0201-positive PBMCs were pulsed 1 hour at 37°C with the native CMV/pp65495-503 peptide (10 -8M), Frontiers in Immunology 04 washed, and incubated with CFSE-labelled (Invitrogen) CMV/ pp65-specific CD8 T cell clones of high or low avidity at an E/T ratio of 1:1 in RPMI 1640 medium (Gibco) supplemented with 8% human serum and 50 U/ml human recombinant IL-2 (GlaxoSmithKline) in the presence of monoclonal IgG2B mouse anti-human LILRB1 (CD85j, ILT2) blocking antibody or an Blocking Assay:Article Title: Overall avidity declines in TCR repertoires during latent CMV but not EBV infection Article Snippet: Ex vivo LILRB1 and CD57 expression along T cell differentiation To assess CD57 and LILRB1 expression along T cell differentiation, CMV/pp65-specific CD8 T cell clonotypes of high versus low avidity isolated and stained as described in the main manuscript (CD8, CD28, CD45RA, CD57, CD85j, CMV/pp65 multimer, Vivid Aqua and appropriate TRBV antibodies (Supplementary Table 1)) were acquired on a Cytoflex flow cytometer (Beckman Coulter) and data were imported in FlowJo software (v.10.4.2, Tree Star) for analysis, using both CD28 and CD45RA as differentiation markers along which LILRB1 and CD57 positive cells were quantified as fractions of parent population. .. CFSE-labelled (Invitrogen) PBMCs from donor BCL4 were pulsed 1 hour at 37°C with native CMV/pp65495-503 peptide (10-8 M) washed and cultured in RPMI 1640 medium (Gibco) supplemented with 8% human serum and 50 U/ml human recombinant IL-2 (GlaxoSmithKline) in the presence of monoclonal IgG2B mouse anti-human LILRB1 (CD85j, ILT-2) blocking antibody or an Article Title: Overall avidity declines in TCR repertoires during latent CMV but not EBV infection. Article Snippet: .. In vitro LILRB1 blocking experiments 30-Gy-irradiated HLA-A*0201-positive PBMCs were pulsed 1 hour at 37°C with the native CMV/pp65495-503 peptide (10 -8M), Frontiers in Immunology 04 washed, and incubated with CFSE-labelled (Invitrogen) CMV/ pp65-specific CD8 T cell clones of high or low avidity at an E/T ratio of 1:1 in RPMI 1640 medium (Gibco) supplemented with 8% human serum and 50 U/ml human recombinant IL-2 (GlaxoSmithKline) in the presence of monoclonal IgG2B mouse anti-human LILRB1 (CD85j, ILT2) blocking antibody or an Control:Article Title: Overall avidity declines in TCR repertoires during latent CMV but not EBV infection Article Snippet: Ex vivo LILRB1 and CD57 expression along T cell differentiation To assess CD57 and LILRB1 expression along T cell differentiation, CMV/pp65-specific CD8 T cell clonotypes of high versus low avidity isolated and stained as described in the main manuscript (CD8, CD28, CD45RA, CD57, CD85j, CMV/pp65 multimer, Vivid Aqua and appropriate TRBV antibodies (Supplementary Table 1)) were acquired on a Cytoflex flow cytometer (Beckman Coulter) and data were imported in FlowJo software (v.10.4.2, Tree Star) for analysis, using both CD28 and CD45RA as differentiation markers along which LILRB1 and CD57 positive cells were quantified as fractions of parent population. .. CFSE-labelled (Invitrogen) PBMCs from donor BCL4 were pulsed 1 hour at 37°C with native CMV/pp65495-503 peptide (10-8 M) washed and cultured in RPMI 1640 medium (Gibco) supplemented with 8% human serum and 50 U/ml human recombinant IL-2 (GlaxoSmithKline) in the presence of monoclonal IgG2B mouse anti-human LILRB1 (CD85j, ILT-2) blocking antibody or an Article Title: Effect of Flavonoids on MCP-1 Expression in Human Coronary Artery Endothelial Cells and Impact on MCP-1-Dependent Migration of Human Monocytes Article Snippet: BD Pharmingen TM Recombinant Human MCP-1 (#554620) was purchased from BD Biosciences (Franklin Lakes, NJ, USA). .. Article Title: Microfluidic-Based Reconstitution of Functional Lymphatic Microvasculature: Elucidating the Role of Lymphatics in Health and Disease. Article Snippet: In a similar set of experiments, Fluorescein-conjugated avidin (Thermofisher) or Alexa Fluor 647-conjugated albumin from bovine serum (Thermofisher) was introduced into the system at a concentration of 100 μg mL−1. .. For neutralizing antibody experiments, PBMCs were incubated in a buffer solution with their respective antibody blocker at a concentration of 5 μg mL−1: human CCR7 antibody (MAB197-SP, R&D), human CXCR4 antibody (MAB172-SP, R&D), and a combine human IgG2A and Article Title: Effect of Flavonoids on MCP-1 Expression in Human Coronary Artery Endothelial Cells and Impact on MCP-1-Dependent Migration of Human Monocytes. Article Snippet: BD PharmingenTM Recombinant Human MCP-1 (#554620) was purchased from BD Biosciences (Franklin Lakes, NJ, USA). .. Article Title: A KLF2-BMPER-Smad1/5 checkpoint regulates high fluid shear stress-mediated artery remodeling Article Snippet: BMPER (Abcam ab73900; IB 1:1000, IF 1:400), KLF4 (Abcam ab215036; IF 1:400). .. Antibodies for BMP9 (MAB3209, clone #360107), BMP10 (MAB2926, clone #462732), IgG2A isotype control (MAB003, clone #20102) and Article Title: Overall avidity declines in TCR repertoires during latent CMV but not EBV infection. Article Snippet: .. In vitro LILRB1 blocking experiments 30-Gy-irradiated HLA-A*0201-positive PBMCs were pulsed 1 hour at 37°C with the native CMV/pp65495-503 peptide (10 -8M), Frontiers in Immunology 04 washed, and incubated with CFSE-labelled (Invitrogen) CMV/ pp65-specific CD8 T cell clones of high or low avidity at an E/T ratio of 1:1 in RPMI 1640 medium (Gibco) supplemented with 8% human serum and 50 U/ml human recombinant IL-2 (GlaxoSmithKline) in the presence of monoclonal IgG2B mouse anti-human LILRB1 (CD85j, ILT2) blocking antibody or an Article Title: A KLF2-BMPER-Smad1/5 checkpoint regulates high fluid shear stress-mediated artery remodeling. Article Snippet: Vascular remodeling to match arterial diameter to tissue requirements commonly fails in ischemic disease.. Endothelial cells sense fluid shear stress (FSS) from blood flow to maintain FSS within a narrow range in healthy vessels.. Thus, high FSS induces vessel outward remodeling, but mechanisms are poorly understood. Incubation:Article Title: Microfluidic-Based Reconstitution of Functional Lymphatic Microvasculature: Elucidating the Role of Lymphatics in Health and Disease. Article Snippet: In a similar set of experiments, Fluorescein-conjugated avidin (Thermofisher) or Alexa Fluor 647-conjugated albumin from bovine serum (Thermofisher) was introduced into the system at a concentration of 100 μg mL−1. .. For neutralizing antibody experiments, PBMCs were incubated in a buffer solution with their respective antibody blocker at a concentration of 5 μg mL−1: human CCR7 antibody (MAB197-SP, R&D), human CXCR4 antibody (MAB172-SP, R&D), and a combine human IgG2A and Article Title: Overall avidity declines in TCR repertoires during latent CMV but not EBV infection. Article Snippet: .. In vitro LILRB1 blocking experiments 30-Gy-irradiated HLA-A*0201-positive PBMCs were pulsed 1 hour at 37°C with the native CMV/pp65495-503 peptide (10 -8M), Frontiers in Immunology 04 washed, and incubated with CFSE-labelled (Invitrogen) CMV/ pp65-specific CD8 T cell clones of high or low avidity at an E/T ratio of 1:1 in RPMI 1640 medium (Gibco) supplemented with 8% human serum and 50 U/ml human recombinant IL-2 (GlaxoSmithKline) in the presence of monoclonal IgG2B mouse anti-human LILRB1 (CD85j, ILT2) blocking antibody or an Concentration Assay:Article Title: Microfluidic-Based Reconstitution of Functional Lymphatic Microvasculature: Elucidating the Role of Lymphatics in Health and Disease. Article Snippet: In a similar set of experiments, Fluorescein-conjugated avidin (Thermofisher) or Alexa Fluor 647-conjugated albumin from bovine serum (Thermofisher) was introduced into the system at a concentration of 100 μg mL−1. .. For neutralizing antibody experiments, PBMCs were incubated in a buffer solution with their respective antibody blocker at a concentration of 5 μg mL−1: human CCR7 antibody (MAB197-SP, R&D), human CXCR4 antibody (MAB172-SP, R&D), and a combine human IgG2A and In Vitro:Article Title: Overall avidity declines in TCR repertoires during latent CMV but not EBV infection. Article Snippet: .. In vitro LILRB1 blocking experiments 30-Gy-irradiated HLA-A*0201-positive PBMCs were pulsed 1 hour at 37°C with the native CMV/pp65495-503 peptide (10 -8M), Frontiers in Immunology 04 washed, and incubated with CFSE-labelled (Invitrogen) CMV/ pp65-specific CD8 T cell clones of high or low avidity at an E/T ratio of 1:1 in RPMI 1640 medium (Gibco) supplemented with 8% human serum and 50 U/ml human recombinant IL-2 (GlaxoSmithKline) in the presence of monoclonal IgG2B mouse anti-human LILRB1 (CD85j, ILT2) blocking antibody or an Clone Assay:Article Title: Overall avidity declines in TCR repertoires during latent CMV but not EBV infection. Article Snippet: .. In vitro LILRB1 blocking experiments 30-Gy-irradiated HLA-A*0201-positive PBMCs were pulsed 1 hour at 37°C with the native CMV/pp65495-503 peptide (10 -8M), Frontiers in Immunology 04 washed, and incubated with CFSE-labelled (Invitrogen) CMV/ pp65-specific CD8 T cell clones of high or low avidity at an E/T ratio of 1:1 in RPMI 1640 medium (Gibco) supplemented with 8% human serum and 50 U/ml human recombinant IL-2 (GlaxoSmithKline) in the presence of monoclonal IgG2B mouse anti-human LILRB1 (CD85j, ILT2) blocking antibody or an |